I. Conformational transition for single GroEL subunit.
Color code: Green for the apical domain, yellow for the intermediate
domain and red for the equatorial domain. The nucleotide is shown by a
space-filling model in blue.
The transition starts from closed form and
goes to the open forms. A total of ten steps (11 frames) are included. It is
important to notice the complexity of the motions, which are "built into" the
single subunit for which the calculation was done but are essential for the
concerted heptamer transition (see second movie).
If the sequence of
motions of the intermediate and apical domain were reversed, a steric clash
would result in the upper hinge. This is the reason that GroEL cannot interact
with GroES before the binding of ATP.
Note: The movie is shown in loop.
The starting point is the ring closed form, i.e., the green apical
domain is down.
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